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KMID : 1024420150190040414
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Food Engineering Progress
2015 Volume.19 No. 4 p.414 ~ p.419
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Identification of an Alkalophilic Bacterium Producing an Alkaline Protease from Soil
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Lee Hyung-Jae
Yoo Ji-Seung
Park Young-Seo
Bai Dong-Hoon
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Abstract
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An alkalophilic microorganism, strain DK1122 producing an alkaline protease was identified. DK1122 was isolated from soil collected in central Korea. The strain DK1122 was Gram-positive, 0.7×2-4 μm in size, and its colony was yellowish white, The strain DK1122 was found to be spore-forming, catalase positive, oxidase positive, caseinolytic, and reduce nitrate to nitrite. The protease was produced aerobically on Horikoshi I agar medium (pH 9.0) with 1% (w/v) skim milk at 40°C for 24 h. Through 16S rRNA gene partial sequencing, the strain DK1122 had the 99.7% sequence similarity to 16S rRNA gene sequence of Bacillus pseudofirmus. Based on the biochemical and physiological properties as well as phylogenetic analysis, the isolated strain was named as Bacillus sp. DK1122. It is expected that Bacillus sp. DK1122 may be a promising candidate for a proudcer of an alkaline protease applicable to the food and detergent industries.
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KEYWORD
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alkaline protease , alkalophilic , Bacillus sp. , identification , soil
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